Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans prmt-5 Polyclonal antibody

Uniprot No.

P46580

Target Names

prmt-5

Alternative Names

prmt-5 antibody; tag-251 antibody; C34E10.5Protein arginine N-methyltransferase 5 antibody; EC 2.1.1.320 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans prmt-5 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the symmetrical dimethylation of arginine residues in targets such as small nuclear ribonucleoproteins, histone H2A/H4 and cbp-1. Dimethylation occurs in a distributive manner where the protein is released after the addition of the first methyl group prior to rebinding for the addition of the second methyl group. Plays a role in the negative regulation of DNA damage-induced apoptosis. By methylating cbp-1, may prevent apoptosis by repressing the capacity of cbp-1 to enhance cep-1 dependent transcription activation of the programmed cell death activator egl-1. Plays a role in heat and oxidative stress resistance.

Gene References into Functions

It show the morphological and functional phenotypes of single or double null alleles of prmt-5 in Caenorhabditis elegans. The prmt-1;prmt-5 double mutants are viable, and exhibit short body length and small brood size compared to N2 and each of the single mutants. PMID: 28158808
The PRMT-5 catalyzes the symmetric dimethylation of substrates containing monomethylarginine residues in vivo. PMID: 28173048
Analysis of prmt-5-deficient worms indicated that methylation promoted the dopamine-mediated modulation of chemosensory and locomotory behaviors through the DOP-3 receptor. PMID: 26554819
data confirm that MEP50 plays a key role in substrate recognition and activates PRMT5 activity by increasing its affinity for protein substrates. PMID: 25485739
the substrate specificity, processivity, and kinetic mechanism of bacterially expressed Caenorhabditis elegans PRMT5 (cPRMT5). PMID: 23866019
analysis of structural insights into protein arginine symmetric dimethylation by PRMT5 PMID: 22143770
PRMT-5 represses CEP-1 transcriptional activity through CBP-1, which represents a novel regulatory mechanism of p53-dependent apoptosis. PMID: 19521535

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Subcellular Location

Nucleus.

Protein Families

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family

Database Links

KEGG: cel:CELE_C34E10.5

STRING: 6239.C34E10.5.3

UniGene: Cel.16951

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prmt-5 Antibody

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