rI Antibody

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Code	CSB-PA320203XA01EDZ
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Target Background

Product Details

Full Product Name

Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) rI Polyclonal antibody

Uniprot No.

P13304

Target Names

Alternative Names

rI antibody; 58.6 antibody; rIA antibody; tk.-2Antiholin antibody; Protein rI antibody

Raised in

Rabbit

Species Reactivity

Enterobacteria phage T4 (Bacteriophage T4)

Immunogen

Recombinant Enterobacteria phage T4 (Bacteriophage T4) rI protein

Immunogen Species

Enterobacteria phage T4 (Bacteriophage T4)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in lysis inhibition. Interacts with and inhibits the holin thereby delaying the host cell lysis timing. Lysis inhibition is imposed when a cell infected by a T4-like virus is superinfected by new incoming viruses. This is probably due to the capsid content of the new phage being released in the periplasm because of blockage of entry due to superinfection exclusion. These periplasmically released viral proteins must somehow activate the periplasmic antiholin, which in turn will block the holin multimerization.

Gene References into Functions

the periplasmic domain of RI is necessary and sufficient to block T-mediated lysis PMID: 16166524

Subcellular Location

Host cell inner membrane; Single-pass type II membrane protein.

Protein Families

T4likevirus antiholin family

Database Links

KEGG: vg:1258591

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Phage Display Service

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rI Antibody

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