AOX4 Antibody

Code CSB-PA687974XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AOX4 Polyclonal antibody
Uniprot No.
Target Names
AOX4
Alternative Names
AOX4; IM; PTOX; At4g22260; T10I14_90; Ubiquinol oxidase 4, chloroplastic/chromoplastic; Alternative oxidase 4; Plastid terminal oxidase; Protein IMMUTANS
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AOX4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Acts early in chloroplast biogenesis as a component of a redox chain responsible for phytoene desaturation. Prevents the generation of toxic oxygen radicals and photooxidation of the nascent photosynthetic apparatus. Involved in the differentiation of multiple plastid types, including chloroplasts, amyloplasts, and etioplasts. Might participate in the chloroplast respiratory chain.
Gene References into Functions
  1. Data indicate that the protein level of plastid terminal oxidase (PTOX) was increased in the bacterial phytoene desaturase (CRTI)-expressing lines. PMID: 24378845
  2. the exon 8 domain of PTOX (IMMUTANS) is required for PTOX activity and stability in Arabidopsis PMID: 16249174
  3. IMMUTANS appears to be a versatile electron sink, especially early in chloroplast development, when its function is crucial for carotenoid biosynthesis, and in excess light, when it serves as a 'safety valve'. PMID: 16449381
  4. Neither functional analyses of the immutans (IM) knockout and overexpression lines nor meta-analyses of gene expression support the model that IM acts as a safety valve to regulate the redox state of the PQ pool during stress and acclimation PMID: 16891546
  5. Plants lacking IM have enhanced rates of carbon assimilation with corresponding increases in the activities of Rubisco and SPS, elevated starch and sucrose pool sizes, and an altered pattern of carbohydrate partitioning that favors sucrose over starch. PMID: 17342448
  6. Lack of colored carotenoids in the im white tissues elicits a differential response from a large number of genes involved in various cellular processes and stress responses. PMID: 19386811
  7. 14 conserved sites fall into three classes: Ala-139, Pro-142, Glu-171, Asn-174, Leu-179, Pro-216, Ala-230, Asp-287, and Arg-293 are dispensable for activity; Tyr-234 and Asp-295 are essential; and Leu-135, His-151, and Tyr-212 are important. PMID: 19542226
  8. In low light, thylakoids from PTOX overexpressed plants were protected against photoinhibition. PMID: 19740740

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Subcellular Location
Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein; Stromal side. Plastid, chromoplast membrane; Multi-pass membrane protein.
Protein Families
Alternative oxidase family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT4G22260

STRING: 3702.AT4G22260.1

UniGene: At.20480

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