AP1 Antibody

Code CSB-PA339569XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AP1 Polyclonal antibody
Uniprot No.
Target Names
AP1
Alternative Names
AP1 antibody; AGL7 antibody; At1g69120 antibody; F4N2.9 antibody; Floral homeotic protein APETALA 1 antibody; Agamous-like MADS-box protein AGL7 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that promotes early floral meristem identity in synergy with LEAFY. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Is indispensable for normal development of sepals and petals in flowers. Regulates positively the B class homeotic proteins APETALA3 and PISTILLATA with the cooperation of LEAFY and UFO. Interacts with SEPALLATA3 or AP3/PI heterodimer to form complexes that could be involved in genes regulation during floral meristem development. Regulates positively AGAMOUS in cooperation with LEAFY. Displays a redundant function with CAULIFLOWER in the up-regulation of LEAFY. Together with AGL24 and SVP, controls the identity of the floral meristem and regulates expression of class B, C and E genes. Represses flowering time genes AGL24, SVP and SOC1 in emerging floral meristems.
Gene References into Functions
  1. APETALA1 establishes determinate floral meristem through regulating cytokinins homeostasis in Arabidopsis. PMID: 26359644
  2. suppression of cytokinin biosynthesis and activation of cytokinin degradation mediates AP1 function in establishing determinate floral meristems PMID: 24753595
  3. Co-expression analysis identifies CRC and AP1 the regulator of Arabidopsis fatty acid biosynthesis. PMID: 22676405
  4. Different action of the APETALA1 gene on the development of reproductive organs in flowers of the abruptus mutant of Arabidopsis thaliana PMID: 21950056
  5. results suggest distinct functions of AP1 during the initiation of flower development PMID: 20360106
  6. The floral homeotic PISTILLATA (PI) protein and its interacting partner APETALA3 directly act, in combination with other factors, to restrict the expression of AP1 during early stages of floral development. PMID: 16640596
  7. AP1, AGL24 and SVP redundantly control floral meristem identity. PMID: 18694458
  8. Angiostatin K1-3 induced E-selectin expression via AP1 and Ets-1 binding to the proximal E-selectin promoter (-356/+1), which was positively mediated by JNK activation. PMID: 18761727

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in young flower primordia, later becomes localized to sepals and petals.
Database Links

KEGG: ath:AT1G69120

STRING: 3702.AT1G69120.1

UniGene: At.10200

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