AP1M2 Antibody

Code CSB-PA991125XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AP1M2 Polyclonal antibody
Uniprot No.
Target Names
AP1M2
Alternative Names
AP1M2 antibody; HAP13 antibody; At1g60780 antibody; F8A5.29AP-1 complex subunit mu-2 antibody; Adaptor protein complex AP-1 subunit mu-2 antibody; Adaptor protein-1 mu-adaptin 2 antibody; Adaptor-related protein complex 1 subunit mu-2 antibody; At-muB2-Ad antibody; Clathrin assembly protein complex 1 mu-2 medium chain antibody; Mu1-adaptin 2 antibody; Protein HAPLESS 13 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AP1M2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting at the trans-Golgi network and early endosomes (TGN/EE). The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Required for KNOLLE localization at the cell plate to mediate cytokinesis. Functions redundantly with AP1M1 in multiple post-Golgi trafficking pathways leading from the TGN to the vacuole, the plasma membrane, and the cell-division plane.
Gene References into Functions
  1. AP-1 complex is required for cell plate-targeted trafficking of KNOLLE in dividing plant cells, and that it has a common role in mediating plant and yeast/animal cytokinesis systems which are fundamentally different. [AP1M2] PMID: 23543752
  2. HAPLESS13 protein is essential for protein sorting at the trans-Golgi network/early endosome. PMID: 23766365
  3. Coimmunoprecipitation revealed that muB2 (At1g60780) is complexed with the large subunit gamma-adaptin of AP-1. PMID: 23733933
Subcellular Location
Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Adaptor complexes medium subunit family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT1G60780

STRING: 3702.AT1G60780.1

UniGene: At.36589

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