AP-2alpha Antibody

Code CSB-PA310880XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) AP-2alpha Polyclonal antibody
Uniprot No.
Target Names
AP-2alpha
Alternative Names
AP-2alpha antibody; CG4260AP-2 complex subunit alpha antibody; Alpha-adaptin antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) AP-2alpha protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP-2alpha is a subunit of the plasma membrane adapter.
Gene References into Functions
  1. The adaptor protein 2 (AP-2) complex binds to a motif in the cytoplasmic tail of Crumbs that overlaps with the binding site of Stardust, a protein known to stabilize Crumbs on the surface. PMID: 26527400
  2. Numb polarizes the distribution of alpha-Adaptin, involved in receptor-mediated endocytosis. Alpha-Adaptin mutations not binding to Numb don't localize asymmetrically and cause defects in asymmetric cell division. PMID: 12194853
  3. Numb and alpha-Adaptin regulate Sanpodo endocytosis to specify cell fate in Drosophila external sensory organs. PMID: 16113648
Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Adaptor complexes large subunit family
Tissue Specificity
Expressed in the Garland cells, imaginal disks, adult midgut precursors, the antenno-maxillary complex, the endoderm, the fat bodies, and the visceral mesoderm and cells of the CNS and PNS including neuroblasts, the presumptive stomatogastric nervous syst
Database Links

KEGG: dme:Dmel_CG4260

STRING: 7227.FBpp0088945

UniGene: Dm.246

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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