AP3 Antibody

Code CSB-PA334008XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AP3 Polyclonal antibody
Uniprot No.
Target Names
AP3
Alternative Names
AP3 antibody; At3g54340 antibody; T12E18_30Floral homeotic protein APETALA 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AP3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Probable transcription factor involved in the genetic control of flower development. Is required for normal development of petals and stamens in the wild-type flower. Forms a heterodimer with PISTILLATA that is required for autoregulation of both AP3 and PI genes. AP3/PI heterodimer interacts with APETALA1 or SEPALLATA3 to form a ternary complex that could be responsible for the regulation of the genes involved in the flower development. AP3/PI heterodimer activates the expression of NAP. AP3/PI prevents GATA22/GNL and GATA21/GNC expression.
Gene References into Functions
  1. Ectopic Fagopyrum esculentum AP3 can rescue stamen development in Arabidopsis ap3 mutant. PMID: 25149019
  2. Data indicate that C function regulator AGAMOUS and the B function regulators APETALA3 and PISTILLATA control many developmental processes in conjunction, as well as independent activities. PMID: 23821642
  3. These results imply considerable changes in the composition and topology of the gene network controlled by AP3/PI during the course of flower development. PMID: 22847437
  4. BNQ genes Are negatively regulated by AP3 and PI in petals. PMID: 20305124
  5. truncated form, lacking the conserved C-terminal motifs, functions to direct floral organ identity specification PMID: 17965182

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in petals and stamens.
Database Links

KEGG: ath:AT3G54340

STRING: 3702.AT3G54340.1

UniGene: At.287

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