AP3BA Antibody

Datasheet

Code	CSB-PA878678XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AP3BA Polyclonal antibody

Uniprot No.

Q9M2T1

Target Names

AP3BA

Alternative Names

AP3BA antibody; PAT2 antibody; At3g55480 antibody; T22E16.140AP3-complex subunit beta-A antibody; Adaptor protein complex AP-3 subunit beta-A antibody; Adaptor-related protein complex 3 subunit beta-A antibody; Beta-3B-adaptin antibody; Clathrin assembly protein complex 3 beta-A large chain antibody; Protein-affected trafficking 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AP3BA protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Part of the AP-3 complex, an adaptor-related complex which seems to be clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. It also function in maintaining the identity of lytic vacuoles and in regulating the transition between storage and lytic vacuoles.

Gene References into Functions

Vacuolar invertases (VIs) might follow an alternative, adaptor protein 3 (AP3)-dependent route in addition to the classical sorting pathway. PMID: 23737500
The phenotypes of the wat1-1D mutant can be explained if loss of function of the AP-3 beta-adaptin (PAT2) causes activation of channels or transporters for organic anions and for K(+) at the plasma membrane. PMID: 23397991
In Arabidopsis plants defective in the beta-subunit of the AP-3 adaptor complex, INT1 is correctly localized to the tonoplast, while sorting of the vacuolar sucrose transporter SUC4 is blocked in cis-Golgi stacks. PMID: 22253225
Evolutionarily conserved AP-3 beta plays a role in the biogenesis and function of the lytic vacuole. PMID: 20729380

Subcellular Location

Cytoplasm. Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Protein Families

Adaptor complexes large subunit family

Database Links

KEGG: ath:AT3G55480

STRING: 3702.AT3G55480.2

UniGene: At.26631

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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AP3BA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background