Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) APY1 Polyclonal antibody

Uniprot No.

Q9SQG2

Target Names

APY1

Alternative Names

APY1 antibody; At3g04080 antibody; T6K12.30Apyrase 1 antibody; AtAPY1 antibody; EC 3.6.1.5 antibody; ATP-diphosphatase antibody; ATP-diphosphohydrolase antibody; Adenosine diphosphatase antibody; ADPase antibody; NTPDase antibody; Nucleoside triphosphate diphosphohydrolase 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana APY1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates. Substrate preference is ATP > ADP. Functions with APY2 to reduce extracellular ATP level which is essential for pollen germination and normal plant development. Plays a role in the regulation of stomatal function by modulating extracellular ATP levels in guard cells.

Gene References into Functions

Study reports that APY1 and, to a lesser extent, APY2 help control root skewing, the right-slanted growth of roots, in Arabidopsis. PMID: 26412783
low levels of apyrase chemical inhibitors can induce stomatal opening in the dark, while apyrase enzyme blocks abscissic acid-induced stomatal closure. PMID: 23989340
APY1, APY2, and extracellular ATP play important roles in the signaling steps that link biotic stresses to plant defense responses and growth changes. PMID: 24550243
Suppression of APY1/APY2 expression results in a significant inhibition of polar auxin transport in Arabidopsis hypocotyls and roots. PMID: 23071251
AtAPY1 is an integral Golgi protein with the substrate specificity typical for Golgi apyrases. PMID: 22849572
AtAPY1 and AtAPY2 are Golgi-localized nucleotide diphosphatases and are likely to have roles in regulating UDP/GDP concentrations in the Golgi lumen. PMID: 23034877
Both short-term inhibition of ectoapyrase activity by polyclonal antibodies and long-term suppression of APY1 and APY2 transcript levels significantly disrupt normal stomatal behavior in light. PMID: 21636723
acts to reduce the concentration of extracellular nucleotides, highest expression in rapidly growing tissues and/or tissues that accumulate high auxin levels PMID: 17434987

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Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein. Membrane; Single-pass type II membrane protein. Note=As cell membrane protein, the functional domain could be at the extracellular side.

Protein Families

GDA1/CD39 NTPase family

Tissue Specificity

Expressed in roots, root hairs, root cap, leaves, stems, trichomes, phloem throughout the plant, guard cells, filaments of young stamens, stipules, papillae of stigmas, pollen, pollen tubes and the abscission zone of siliques.

Database Links

KEGG: ath:AT3G04080

STRING: 3702.AT3G04080.1

UniGene: At.16940

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APY1 Antibody

Product Details

Customer Reviews and Q&A

Target Background