ARC1 Antibody

Code CSB-PA342021XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ARC1 Polyclonal antibody
Uniprot No.
Target Names
ARC1
Alternative Names
ARC1 antibody; G4P1 antibody; YGL105W antibody; G3085 antibody; tRNA-aminoacylation cofactor ARC1 antibody; Acyl-RNA-complex protein 1 antibody; GU4 nucleic-binding protein 1 antibody; G4p1 protein antibody; P42 antibody; tRNA-interacting factor ARC1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ARC1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Binds to tRNA and functions as a cofactor for the methionyl-tRNA synthetase (MetRS) and glutamyl-tRNA synthetase (GluRS). Forms a complex with MetRS and GluRS and increases their affinity for cognate tRNAs due to the presence of a tRNA binding domain in its middle and C-terminal part. Binds specifically G4 quadruplex nucleic acid structures (these are four-stranded right-handed helices, stabilized by guanine base quartets). Also required for cytoplasmic confinement of the synthetases and tRNA.
Gene References into Functions
  1. study suggests that the structure and function of Arc1p can be modulated via biotinylation in response to temperature changes. PMID: 27330079
  2. An ab initio model of both trimeric and the pentameric complexes containing Arc1,GluRS and MetRS. PMID: 23161686
  3. Present study suggests a more complex picture for Arc1p-mediated stimulation of yeast tRNA glutamylation than just a role in increasing the glutamyl-tRNA synthetase binding affinity for tRNAGlu. PMID: 15667228
  4. cytoplasmic location of Arc1p is maintained by Xpo1p-dependent nuclear export PMID: 15710377
  5. dual localization of (c)ERS is controlled by binding to Arc1p PMID: 19417106

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Subcellular Location
Cytoplasm. Note=Largely excluded from the nucleus.
Protein Families
TRNA-aminoacylation cofactor ARC1 family
Database Links

KEGG: sce:YGL105W

STRING: 4932.YGL105W

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