arcB Antibody

Code CSB-PA365123XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) arcB Polyclonal antibody
Uniprot No.
Target Names
arcB
Alternative Names
arcB; b3210; JW5536; Aerobic respiration control sensor protein ArcB
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) arcB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Member of the two-component regulatory system ArcB/ArcA. Sensor-regulator protein for anaerobic repression of the arc modulon. Activates ArcA via a four-step phosphorelay. ArcB can also dephosphorylate ArcA by a reverse phosphorelay involving His-717 and Asp-576.
Gene References into Functions
  1. The authors observed that wild-type, DeltaarcA, and DeltaarcB had comparable initial NO* clearance times; however, the mutant cultures failed to exhibit [NO*] and [O2 ] oscillations. these results establish ArcAB as a TCS that influences NO* detoxification in E. coli within the physiologically-relevant microaerobic regime. PMID: 28449226
  2. Data show that the redox potential of ArcB is about -41 mV, and we demonstrate that the menaquinols are required for proper ArcB activation upon a shift from aerobic to anaerobic growth conditions. PMID: 23645604
  3. These analyses revealed that elimination of different segments in ArcB determines a distinctive distribution of d-glucose catabolic fluxes, different from that observed in the DeltaarcB background. PMID: 23064346
  4. The results lead to the working hypothesis that the in vivo activity of ArcB in E. coli is modulated by the redox state of the menaquinone pool and that the ubiquinone/ubiquinol ratio in vivo surely is not the only determinant of ArcB activity. PMID: 19933363
  5. the ArcB/ArcA/RssB system is involved in sigma(S) induction during entry into starvation conditions. PMID: 16291649
  6. An active form of ArcB promotes low-level induction of psp expression independent of pIV and communicates with PspB. PMID: 19682256
  7. Both ArcA and ArcB were necessary for resistance to hydrogen peroxide, and their function in this resistance was independent from H2O2 scavenge. PMID: 19715602

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Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Database Links
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