ARF2 Antibody

Code CSB-PA856741XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF2 Polyclonal antibody
Uniprot No.
Target Names
ARF2
Alternative Names
ARF2 antibody; MNT antibody; At5g62000 antibody; MTG10.3Auxin response factor 2 antibody; ARF1-binding protein antibody; ARF1-BP antibody; Protein MEGAINTEGUMENTA antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARF2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Promotes flowering, stamen development, floral organ abscission and fruit dehiscence. Functions independently of ethylene and cytokinin response pathways. May act as a repressor of cell division and organ growth.
Gene References into Functions
  1. ARF2 and HB33 are novel regulators in the abscissic acid signal pathway, which has crosstalk with auxin signal pathway in regulating plant growth. PMID: 21779177
  2. Data suggest that ARF2 positively regulates leaf senescence in Arabidopsis. PMID: 20164142
  3. ARF2 is a part of transcriptional complexes responsible for regulating diverse signaling pathways leading to pleiotropic developmental defects. [ARF2][Auxin Response Factor 2] PMID: 15960614
  4. ARF2 expression was induced in senescing leaves. ARF2 regulated leaf senescence and floral organ abscission independently of the ethylene and cytokinin response pathways PMID: 16176952
  5. mutant phenotype and gene expression studies provide evidence that MNT/ARF2 is a repressor of cell division and organ growth PMID: 16339187
  6. study describes a direct connection between the brassinosteroid-regulated BIN2 kinase and ARF2; phosphorylation by BIN2 results in loss of ARF2 DNA binding and repression activities PMID: 18599455

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Subcellular Location
Nucleus.
Protein Families
ARF family
Tissue Specificity
Expressed in the whole plant.
Database Links

KEGG: ath:AT5G62000

STRING: 3702.AT5G62000.1

UniGene: At.21704

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