ARF5 Antibody

Code CSB-PA309745XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF5 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
ARF5 antibody; IAA24 antibody; MP antibody; At1g19850 antibody; F6F9.10 antibody; Auxin response factor 5 antibody; Auxin-responsive protein IAA24 antibody; Transcription factor MONOPTEROS antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARF5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity.
Gene References into Functions
  1. MP plays a critical role in Arabidopsis embryonic root initiation. PMID: 28265057
  2. activating a steroid-inducible variant of the auxin response factor (ARF) MONOPTEROS (MP) is sufficient to restore patterning and PIN gene expression. PMID: 27441727
  3. These findings reveal a patterning module in plants that determines organ position by orienting transport of the hormone auxin toward cells with high levels of MP-mediated auxin signaling. PMID: 27818174
  4. A report or the crystal structure of ARF5 domain III/IV and the molecular determinants of ARF-IAA interactions. PMID: 24710426
  5. It was concluded that the MPDelta genotype can promote de novo shoot formation and can be used to probe corresponding signaling pathways. PMID: 25274430
  6. The involvement of ARF5 in the transcriptional regulation of the entire Aux/IAA family in Arabidopsis thaliana was studied. PMID: 25145395
  7. IAA17 and ARF5 associate to form homo- or hetero-oligomers using a common scaffold and binding interfaces, but their affinities vary significantly PMID: 25512488
  8. Characterization of Columbia alleles of mp, shows four new alleles among which one is a low-expression allele of mp and the another the strongest Columbia allele of mp. PMID: 24281793
  9. Study shows that ARF DNA-binding domains ahomodimerize to generate cooperative DNA binding, which is critical for in vivo ARF5/MP function. Strikingly, DNA-contacting residues are conserved between ARFs, and found that monomers have the same intrinsic specificity; ARF1 and ARF5 homodimers, however, differ in spacing tolerated between binding sites. PMID: 24485461
  10. MP mutant plant exhibits an adaxial-abaxial asymmetry in its ability to influence organ development. PMID: 22751359
  11. A semi-dominant, gain-of-function allele of MONOPTEROS is identified, which results in vein proliferation of leaves and cotyledons. PMID: 22349732
  12. MP with the domains III and IV eliminated can selectively uncouple a single ARF from regulation by Aux/IAA proteins. PMID: 22320407
  13. TARGET OF MP 5 (TMO5) and TMO7 encode basic helix-loop-helix (bHLH) transcription factors that are expressed in the hypophysis-adjacent embryo cells, and are required and partially sufficient for MONOPTEROS-dependent root initiation PMID: 20220754
  14. expression can be activated by auxin exposure PMID: 17217464
  15. MONOPTEROS promotes the focusing of auxin and leaf initiation in part through pathways not affected by auxin efflux inhibitors. PMID: 18685044

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Subcellular Location
Nucleus.
Protein Families
ARF family
Tissue Specificity
Expressed in the whole plant with a lower expression in leaves. Detected in embryo axis, provascular tissues, procambium and some differentiated vascular regions of mature organs.
Database Links

KEGG: ath:AT1G19850

STRING: 3702.AT1G19850.1

UniGene: At.10494

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