Recombinant Arabidopsis thaliana Auxin response factor 5 (ARF5), partial

Code CSB-YP309745DOA
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Source Yeast
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Code CSB-EP309745DOA
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Source E.coli
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Code CSB-EP309745DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP309745DOA
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Source Baculovirus
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Code CSB-MP309745DOA
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
ARF5; IAA24; MP; At1g19850; F6F9.10; Auxin response factor 5; Auxin-responsive protein IAA24; Transcription factor MONOPTEROS
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity.
Gene References into Functions
  1. MP plays a critical role in Arabidopsis embryonic root initiation. PMID: 28265057
  2. activating a steroid-inducible variant of the auxin response factor (ARF) MONOPTEROS (MP) is sufficient to restore patterning and PIN gene expression. PMID: 27441727
  3. These findings reveal a patterning module in plants that determines organ position by orienting transport of the hormone auxin toward cells with high levels of MP-mediated auxin signaling. PMID: 27818174
  4. A report or the crystal structure of ARF5 domain III/IV and the molecular determinants of ARF-IAA interactions. PMID: 24710426
  5. It was concluded that the MPDelta genotype can promote de novo shoot formation and can be used to probe corresponding signaling pathways. PMID: 25274430
  6. The involvement of ARF5 in the transcriptional regulation of the entire Aux/IAA family in Arabidopsis thaliana was studied. PMID: 25145395
  7. IAA17 and ARF5 associate to form homo- or hetero-oligomers using a common scaffold and binding interfaces, but their affinities vary significantly PMID: 25512488
  8. Characterization of Columbia alleles of mp, shows four new alleles among which one is a low-expression allele of mp and the another the strongest Columbia allele of mp. PMID: 24281793
  9. Study shows that ARF DNA-binding domains ahomodimerize to generate cooperative DNA binding, which is critical for in vivo ARF5/MP function. Strikingly, DNA-contacting residues are conserved between ARFs, and found that monomers have the same intrinsic specificity; ARF1 and ARF5 homodimers, however, differ in spacing tolerated between binding sites. PMID: 24485461
  10. MP mutant plant exhibits an adaxial-abaxial asymmetry in its ability to influence organ development. PMID: 22751359
  11. A semi-dominant, gain-of-function allele of MONOPTEROS is identified, which results in vein proliferation of leaves and cotyledons. PMID: 22349732
  12. MP with the domains III and IV eliminated can selectively uncouple a single ARF from regulation by Aux/IAA proteins. PMID: 22320407
  13. TARGET OF MP 5 (TMO5) and TMO7 encode basic helix-loop-helix (bHLH) transcription factors that are expressed in the hypophysis-adjacent embryo cells, and are required and partially sufficient for MONOPTEROS-dependent root initiation PMID: 20220754
  14. expression can be activated by auxin exposure PMID: 17217464
  15. MONOPTEROS promotes the focusing of auxin and leaf initiation in part through pathways not affected by auxin efflux inhibitors. PMID: 18685044

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Subcellular Location
Protein Families
ARF family
Tissue Specificity
Expressed in the whole plant with a lower expression in leaves. Detected in embryo axis, provascular tissues, procambium and some differentiated vascular regions of mature organs.
Database Links

KEGG: ath:AT1G19850

STRING: 3702.AT1G19850.1

UniGene: At.10494

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