Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF7 Polyclonal antibody

Uniprot No.

P93022

Target Names

ARF7

Alternative Names

ARF7 antibody; BIP antibody; IAA21 antibody; IAA23 antibody; IAA25 antibody; NPH4 antibody; TIR5 antibody; At5g20730 antibody; T1M15.130 antibody; Auxin response factor 7 antibody; Auxin-responsive protein IAA21/IAA23/IAA25 antibody; Protein BIPOSTO antibody; Protein NON-PHOTOTROPIC HYPOCOTYL 4 antibody; Protein TRANSPORT INHIBITOR RESPONSE 5 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ARF7 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Acts as a transcriptional activator of several tropic stimulus-induced (TSI) genes, including SAUR50. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Required for differential growth responses of aerial tissues. Involved in ethylene responses. Regulates lateral root formation through direct regulation of LBD16 and/or LBD29. Functionally redundant with ARF19. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF5.

Gene References into Functions

ARF7 and FLP transcription factors jointly form a coherent feed-forward motif that mediates the auxin-responsive PIN3 transcription in planta to steer the early steps of lateral root formation. PMID: 26578065
Analyses of the expression pattern of ARF7 and ARF5 targets suggest that this patterning mechanism controls flanking and central zone specification in Arabidopsis LR primordia. PMID: 25944102
the thermodynamic and structural basis for Arabidopsis thaliana ARF7 PB1 domain self-interaction. PMID: 25839233
Mutations affecting either ARF7 or ARF19 function almost fully blocked manifestation of the sar1-7-dependent ethylene hypersensitivity phenotype. PMID: 22238449
ARF7 plays a major role in regulating expression of a subset of auxin response genes in leaf mesophyll cells. PMID: 15923351
Mutations in ARF19 have little effect on their own, but in combination with mutations in NPH4/ARF7, encoding the most closely related ARF, they cause several phenotypes. PMID: 15960621
ARF7 acts to regulate the auxin-mediated transcription of LATERAL ORGAN BOUNDARIES-DOMAIN16/ASYMMETRIC LEAVES2-LIKE18 (LBD16/ASL18) and/or LBD29/ASL16 in roots. PMID: 17259263

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Subcellular Location

Nucleus.

Protein Families

ARF family

Tissue Specificity

Expressed in the whole plant.

Database Links

KEGG: ath:AT5G20730

STRING: 3702.AT5G20730.1

UniGene: At.10493

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ARF7 Antibody

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