ARF10 Antibody

Code CSB-PA882894XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARF10 Polyclonal antibody
Uniprot No.
Target Names
ARF10
Alternative Names
ARF10 antibody; At2g28350 antibody; T1B3.13Auxin response factor 10 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARF10 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Could act as transcriptional activator or repressor. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression.
Gene References into Functions
  1. Auxin recruits AUXIN RESPONSE FACTOR 10 and AUXIN RESPONSE FACTOR 16 to control the expression of ABI3 during seed germination. PMID: 23986496
  2. miR160 negatively affects the expression of ARF10 during shoot regeneration. PMID: 22335436
  3. Phenotypic plasticity of adventitious rooting is controlled by complex regulation of AUXIN RESPONSE FACTOR transcripts and microRNA abundance. Repressor/Activator Balance Is Unaltered by Disruptions to ARF10 and ARF16. PMID: 19820192
  4. In root, auxin and miR160 regulate the expression of ARF10 genes independently, generating a pattern consistent with root cap development. PMID: 16006581
  5. negative regulation of ARF10 by miR160 plays a critical role in seed germination and post-embryonic developmental programs PMID: 17672844

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Subcellular Location
Nucleus.
Protein Families
ARF family
Tissue Specificity
Expressed in the whole plant.
Database Links

KEGG: ath:AT2G28350

STRING: 3702.AT2G28350.1

UniGene: At.13730

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