argP Antibody

Datasheet

Code	CSB-PA359182XA01ENV
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) argP Polyclonal antibody

Uniprot No.

P0A8S1

Target Names

argP

Alternative Names

argP antibody; iciA antibody; b2916 antibody; JW2883HTH-type transcriptional regulator ArgP antibody; Inhibitor of chromosome initiation antibody; OriC replication inhibitor antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) argP protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Controls the transcription of genes involved in arginine and lysine metabolism. Activates transcription of several genes, including argO, lysP, lysC, asd, dapB, dapD, lysA, gdhA and argK. Acts by binding directly to their promoter or control region. ArgP dimer by itself is able to bind the argO promoter-operator region to form a binary complex, but the formation of a ternary complex with RNA polymerase is greatly stimulated only in presence of a coeffector. Both arginine and lysine are coeffectors at the argO promoter, but only arginine is competent to activate transcription. Lysine has repressive effects. ArgP also mediates lysine repression of dapB, and gdhA in vivo, but via an alternative mechanism: ArgP binding is directly reduced upon the addition of lysine. Binds in vitro to the promoter region of dnaA and to the upstream region of the nrd promoter, but these genes are probably not regulated by ArgP in vivo. In vitro, binds also to the three 13-mers located in the origin region (oriC) and blocks the initiation of replication.

Gene References into Functions

ArgP is a transcriptional regulator for lysine repression of genes and it is noncanonical in that it also exhibits low-affinity binding, without apparent direct regulatory effect, to a number of additional sites in the genome. PMID: 21890697
These results indicate that ArgP is a major regulator of lysP expression but that Lrp modulates lysP transcription under lysine-limiting conditions. PMID: 21441513
Activation by Lrp interferes with the previously demonstrated activation of the argO promoter by ArgP. PMID: 19906180
The ArgP dimer by itself is able to bind the argO promoter-operator region to form a binary complex, but that the formation of a ternary complex with RNA polymerase is greatly stimulated only in presence of a coeffector. PMID: 17504942

Database Links

KEGG: ecj:JW2883

STRING: 316385.ECDH10B_3091

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

argP Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background