ARI12 Antibody

Code CSB-PA774608XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARI12 Polyclonal antibody
Uniprot No.
Target Names
ARI12
Alternative Names
ARI12 antibody; At1g05880 antibody; T20M3.15 antibody; Probable E3 ubiquitin-protein ligase ARI12 antibody; EC 2.3.2.31 antibody; ARIADNE-like protein ARI12 antibody; Protein ariadne homolog 12 antibody; RING-type E3 ubiquitin transferase ARI12 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARI12 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.
Gene References into Functions
  1. ARI12 is a member of a gene family in Arabidopsis of 16 genes including two pseudogenes and mainly expressed in roots. PMID: 12529512
  2. ARI12 is a functional RING-HECT E3 ubiquitin ligase which is mono-ubiquitinated. ARI12 transcript and protein abundance depends on COP1 in white light, low and high fluence rates of UV-B. PMID: 25817546
  3. ARI12 is a downstream target of the UV-B receptor, UVR8, and the transcription factors HY5 and HYH under low fluence rates. Under high fluence rates of broad band UV-B/A ARI12 transcription is suppressed by UVR8 and the blue light/UV-A receptors cryptochromes 1 and 2. ARI12 expression is independent of phototropin 1 and 2 and phytochomes A and B. PMID: 22990446
Protein Families
RBR family, Ariadne subfamily
Tissue Specificity
Preferentially expressed in roots.
Database Links

KEGG: ath:AT1G05880

STRING: 3702.AT1G05880.2

UniGene: At.49860

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