ARR12 Antibody

Code CSB-PA352929XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARR12 Polyclonal antibody
Uniprot No.
Target Names
ARR12
Alternative Names
ARR12 antibody; At2g25180 antibody; F13D4.140Two-component response regulator ARR12 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARR12 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. Involved in the root-meristem size determination through the regulation of cell differentiation. Involved in activating SHY2 during meristem growth and controls PIN expression via activation of SHY2.
Gene References into Functions
  1. The cytokinin module ARABIDOPSIS HISTIDINE KINASE3 (AHK3)/ARABIDOPSIS RESPONSE REGULATOR1 (ARR1)/ARR12 was found to interact with the GR24-dependent reduction in LR development, because mutants in this pathway rendered LR development insensitive to GR24. PMID: 26519957
  2. An arr1,10,12 triple mutant had more drought tolerance due to enhanced cell membrane integrity, anthocyanin synthesis, and abscissic acid hypersensitivity, and reduced stomatal aperture. Dehydration and ABA repressed all 3 genes. PMID: 26884175
  3. low temperature inhibits root growth by reducing auxin accumulation via ARR1/12. PMID: 25552473
  4. Cytokinin regulates sodium homeostasis via ARR1 and ARR12, and acts through ARR1 and ARR12 to regulate AtHKT1;1 expression. PMID: 21105923
  5. ARR10 and ARR12play pivotal roles in the histidine kinase-dependent phosphorelay signaling in response to cytokinin in roots. PMID: 17132632
  6. The results of this study suggested that ARR1, ARR10 and ARR12 together play essential (or general) roles in cytokinin signal transduction. PMID: 18037673
  7. Results support a model in which cytokinin regulates a wide array of downstream responses through the action of a multistep phosphorelay that culminates in transcriptional regulation by ARR1, ARR10, and ARR12. PMID: 18723577

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Subcellular Location
Nucleus.
Protein Families
ARR family, Type-B subfamily
Tissue Specificity
Detected in the whole plant. Predominantly expressed in leaves. Expressed at the root transition zone.
Database Links

KEGG: ath:AT2G25180

STRING: 3702.AT2G25180.1

UniGene: At.19775

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