AS1 Antibody

Code CSB-PA528050XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AS1 Polyclonal antibody
Uniprot No.
Target Names
AS1
Alternative Names
AS1 antibody; MYB91 antibody; PHAN antibody; At2g37630 antibody; F13M22.13Transcription factor AS1 antibody; Myb-related protein 91 antibody; AtMYB91 antibody; Protein ASYMMETRIC LEAVES 1 antibody; Protein PHANTASTICA antibody; AtPHAN antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AS1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor required for normal cell differentiation. Positively regulates LATERAL ORGAN BOUNDARIES (LOB) within the shoot apex, and the class III HD-ZIP genes REV, PHB, and PHV. Interacts directly with ASYMMETRIC LEAVES 2 (LBD6/AS2) to repress the knox homeobox genes BP/KNAT1, KNAT2, and KNAT6 and the abaxial determinants ARF3/ETT, KAN2 and YAB5. May act in parallel with the RDR6-SGS3-AGO7 pathway, an endogenous RNA silencing pathway, to regulate the leaf morphogenesis. Binds directly to KNAT1, KNAT2, and KNATM chromatin, regulating leaf development. LBD6 is required for this binding. Positive regulator of flowering that binds to the promoter of FT. Regulates FT expression by forming a functional complex with CO. Involved in leaf polarity establishment by functioning cooperatively with NUCL1 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures.
Gene References into Functions
  1. that complexes of the transcription factors ASYMMETRIC LEAVES 1 (AS1) and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS and KNAT2 via direct interactions with LHP1. PMID: 27273574
  2. critical for the proper placement of the floral organ abscission zones, and influences the timing of organ shedding PMID: 25038814
  3. CONSTANS (CO) forms a functional complex with ASYMMETRIC LEAVES 1 (AS1) to regulate FLOWERING LOCUS T (FT) expression and that AS1 plays different roles in two regulatory pathways, both of which concomitantly regulate the precise timing of flowering. PMID: 21950734
  4. Negative transcriptional, post-transcriptional and epigenetic regulation of ARF3 by AS1-AS2 is important for stabilizing early leaf partitioning into abaxial and adaxial domains. PMID: 23571218
  5. Taken together, these data indicate that HDA6 is a part of the AS1 repressor complex to regulate the KNOX expression in leaf development. PMID: 23271976
  6. AS1 transcriptional control of meristem cell-specific genes is inhibited by Calmodulin. PMID: 22554014
  7. The JLO and AS2 proteins interact molecularly and form multimeric complexes with AS1 to suppress KNOX expression. AS2 together with JLO regulate auxin transport in seedling roots. PMID: 22822207
  8. In combination with mutations in the MYB domain transcription factor gene ASYMMETRIC LEAVES1 (AS1), partial loss of EMBRYO DEFECTIVE DEVELOPMENT1 (EDD1) function results in leaves with reduced adaxial fate. PMID: 22791832
  9. Data show that TCP3 directly activates the expression of genes for miR164, ASYMMETRIC LEAVES1 (AS1), INDOLE-3-ACETIC ACID3/SHORT HYPOCOTYL2 (IAA3/SHY2), and SMALL AUXIN UP RNA (SAUR) proteins. PMID: 21119060
  10. Results revealed that the reduction in leaf size and late flowering were caused by the repression, by KNOX genes, of a gibberellin (GA) pathway in as1 and as2 plants. PMID: 19891706
  11. Our data suggest that RS2/AS1 and HIRA mediate the epigenetic silencing of knox genes, possibly by modulating chromatin structure. PMID: 16243907
  12. RDR6, SGS3 and AGO7 act in the same pathway, which genetically interacts with the AS1-AS2 pathway for leaf development. PMID: 16699177
  13. AS1 patterns the Arabidopsis gynoecium by repressing BP. PMID: 17592013
  14. function of AS1 in responses to phytopathogens is independent of its AS2-associated role in development PMID: 18003921
  15. A previously unrecognized fundamental regulation by which AS1, AS2, and JAG act to define sepal and petal from their boundaries is reported. PMID: 18156293
  16. AS1 and AS2 form a repressor complex that binds directly to the regulatory motifs CWGTTD and KMKTTGAHW present at two sites in the promoters of the KNOX genes BREVIPEDICELLUS (BP) and KNAT2. PMID: 18203921
  17. results of studying new alleles of AS1 and AS2 support their role in control of class I KNOX genes and auxin transport. PMID: 18409376
  18. AS1, AS2 and the AS1-AS2 protein complex may have distinct functions, which are all required for normal plant development. PMID: 18713400

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, stems, leaves, flowers, siliques and in lateral organ promordia. Found in the inner domain between the adaxial and abaxial domains of leaves. Expressed in the phloem tissues of leaves, cotyledons, hypocotyls, and roots.
Database Links

KEGG: ath:AT2G37630

STRING: 3702.AT2G37630.1

UniGene: At.11577

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