asd-2 Antibody

Code CSB-PA267656XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans asd-2 Polyclonal antibody
Uniprot No.
Target Names
asd-2
Alternative Names
asd-2 antibody; let-529 antibody; star-2 antibody; tag-44 antibody; T21G5.5RNA-binding protein asd-2 antibody; Alternative splicing defective protein 2 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans asd-2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
RNA-binding protein that binds to the 5'-NACUAAY-N(1,20)-UAAY-3' consensus sequence in pre-mRNA introns to promote alternative splicing. Required for mutually exclusive alternative splicing where it modulates the switch between mutually exclusive exons during pre-mRNA maturation. Involved in muscle-specific gene expression regulating the alternative splicing of genes such as let-2 and unc-60 to ensure that their respective isoforms are expressed in muscle. Promotes the removal of intron 10 from let-2 pre-mRNA to allow for the exclusive expression of the muscle-specific let-2 isoform (as opposed to the non-muscle-specific isoform expressed in embryos) in body wall muscles during late larval and adult stages of development. Binds cooperatively with RNA-binding protein sup-12 to intron 1A of the unc-60 pre-mRNA to promote alternative splicing and expression of the muscle specific isoform of unc-60.
Gene References into Functions
  1. We isolated several switching-defective mutants and identified the alternative splicing defective-2 (asd-2) gene, encoding a novel member of the evolutionarily conserved STAR (signal transduction activators of RNA) family of RNA-binding proteins. PMID: 18230701
Subcellular Location
Nucleus.
Tissue Specificity
Isoform b: Expressed in the hypodermis and pharyngeal muscles. Isoform c: Expressed in body wall muscles and phayngeal muscles.
Database Links

UniGene: Cel.16725

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