Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ASH1 Polyclonal antibody

Uniprot No.

P34233

Target Names

ASH1

Alternative Names

ASH1 antibody; YKL185WTranscriptional regulatory protein ASH1 antibody; Daughter cells HO repressor protein antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ASH1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Component of the RPD3C(L) histone deacetylase complex (HDAC). Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. ASH1 is necessary to repress HO in daughter cells to block mating-type switching through its binding to HO promoter 5'-YTGAT-3' sites. Also involved in pseudohyphal growth.

Gene References into Functions

Three X-ray structures of the ASH1-E3 localization element alone, in complex with its nuclear RBP She2p, and in the cytoplasmic synergistic ternary complex with She2p and She3p. PMID: 28092367
ASH1 messenger ribonucleoprotein-core factors form stable complexes in absence of cargo RNA at physiological conditions. PMID: 25826656
Recent advances have helped to draw an increasingly clear picture on the molecular mechanisms governing ASH1 mRNA localization from its co-transcriptional birth to its delivery at the site of destination PMID: 25482892
ASH1 mRNA is transported to the bud tip by the class V myosin Myo4. PMID: 20457760
We assessed ASH1 mRNA and Myo4p distribution more directly and found that their localization is impaired in cells expressing globular tail-lacking Myo4p. PMID: 20439999
sequence-specific repressors, Ash1 is stably associated with Rpd3L PMID: 16314178
the translation initiation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction PMID: 18413716
Data show that ASH1 mRNA and She2 localize specifically to the nucleolus when their nuclear export is blocked. PMID: 18566598

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Subcellular Location

Nucleus. Note=Preferentially accumulates in daughter cell nuclei at the end of anaphase.

Database Links

KEGG: sce:YKL185W

STRING: 4932.YKL185W

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ASH1 Antibody

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