ASI1 Antibody

Code CSB-PA911628XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ASI1 Polyclonal antibody
Uniprot No.
Target Names
ASI1
Alternative Names
ASI1 antibody; IBM2 antibody; SG2 antibody; At5g11470 antibody; F15N18_60Protein ANTI-SILENCING 1 antibody; Protein INCREASE IN BONSAI METHYLATION 2 antibody; Protein SHOOT GROWTH 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ASI1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Required to prevent promoter DNA hypermethylation and transcriptional silencing of some transgenes. Plays a similar role to that of the histone H3K9 demethylase JMJ25/IBM1 in preventing CHG methylation in the bodies of numerous genes. RNA-binding protein that ensures the proper expression of JMJ25/IBM1 full-length transcript by associating with an intronic heterochromatic repeat element of JMJ25/IBM1. May associate with intronic heterochromatin and bind gene transcripts to promote their 3' distal polyadenylation. Contributes to a unique mechanism to deal with the collateral effect of silencing intronic repeat elements.
Gene References into Functions
  1. our results suggest a function for SG1 in the maintenance of genome integrity by regulating IBM1. [SG1] PMID: 24404182
  2. ASI1 associates with intronic heterochromatin and binds the gene transcripts to promote their 3' distal polyadenylation. [ASI1] [ANTI-SILENCING 1] PMID: 24003136
  3. In Arabidopsis, full-length transcript formation over intragenic heterochromatin depends on a protein named IBM2 (Increase in Bonsai Methylation 2), which has a Bromo-Adjacent Homology domain and an RNA recognition motif. [IBM2] PMID: 23934508
Database Links

KEGG: ath:AT5G11470

STRING: 3702.AT5G11470.1

UniGene: At.54819

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