asiA Antibody

Datasheet

Code	CSB-PA327122XA01EDZ
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Target Background

Product Details

Full Product Name

Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) asiA Polyclonal antibody

Uniprot No.

P32267

Target Names

asiA

Alternative Names

asiA antibody; 10 kDa anti-sigma factor antibody; 10 kDa RNA polymerase-associated protein antibody; Audrey Stevens' inhibitor antibody

Raised in

Rabbit

Species Reactivity

Enterobacteria phage T4 (Bacteriophage T4)

Immunogen

Recombinant Enterobacteria phage T4 (Bacteriophage T4) asiA protein

Immunogen Species

Enterobacteria phage T4 (Bacteriophage T4)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.

Gene References into Functions

The authors show that AsiA also plays a direct role in T4 middle promoter activation by contacting the MotA activation domain. PMID: 19843221
AsiA simultaneously occupies the surface of region 4 that ordinarily contacts core RNA polymerase (RNAP) PMID: 15257291
sequence comparisons of T4 AsiA with Rsd, indicate that these proteins share conserved amino acid residues at positions important for the binding of T4 AsiA to sigma70 region 4 PMID: 15561138
AsiA protein plays a key role in T4 development through its ability to both inhibit and activate bacterial RNA polymerase transcription. Review. PMID: 15802215
review summarizes interactions of AsiA and MotA with sigma(70) and discusses how these interactions accomplish the switch to T4 middle promoters [review] PMID: 15941982

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Database Links

KEGG: vg:1258709

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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asiA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background