Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG18 Polyclonal antibody

Uniprot No.

P43601

Target Names

ATG18

Alternative Names

ATG18 antibody; AUT10 antibody; CVT18 antibody; NMR1 antibody; SVP1 antibody; YFR021W antibody; Autophagy-related protein 18 antibody; Cytoplasm to vacuole targeting protein 18 antibody; Needed for premeiotic replication protein 1 antibody; Swollen vacuole phenotype protein 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG18 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). May negatively regulate FAB1 activity by sequestering or masking VAC7 from FAB1. Necessary for proper vacuole morphology. Plays an important role in osmotically-induced vacuole fragmentation. Required for cytoplasm to vacuole transport (Cvt) vesicle formation, pexophagy and starvation-induced autophagy. Involved in correct ATG9 trafficking to the pre-autophagosomal structure. Might also be involved in premeiotic DNA replication. With ATG2, protects ATG8 from ATG4-mediated cleavage.

Gene References into Functions

Atg2-Atg18 complex tethers the preautophagosomal structure to the endoplasmic reticulum to initiate membrane expansion during autophagosome formation. PMID: 30254161
Data provide a model whereby the Atg18 beta-propeller provides organelle specificity by binding to two determinants on the target membrane. PMID: 23230146
The Atg2-Atg18 complex and Atg9 localized to the edge of the IM, appearing as two or three dots, in close proximity to the endoplasmic reticulum exit sites. PMID: 23549786
effective targeting of Atg2 to the PAS can compensate for loss of Atg18 function in autophagy PMID: 22728243
Svp1p is a PtdIns(3,5)P2 effector required for PtdIns(3,5)P2-dependent membrane recycling from the vacuole. PMID: 15103325
Cvt pathway requires binding of Atg18 to PtdIns3P PMID: 16876790
Atg18 forms a complex with Atg2 irrespective of PtdIns(3)P binding, associates tightly to autophagic membranes by interacting with PtdIns(3)P, and plays an essential role. PMID: 18586673
Deletion of ATG18, ATG21 and YGR223c, alone or simultaneously has no obvious function on the MVB-pathway and carboxypeptidase Y sorting. PMID: 18769150

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Subcellular Location

Preautophagosomal structure membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein.

Protein Families

WD repeat SVP1 family

Database Links

KEGG: sce:YFR021W

STRING: 4932.YFR021W

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ATG18 Antibody

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