ATG21 Antibody

Code CSB-PA311630XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG21 Polyclonal antibody
Uniprot No.
Target Names
ATG21
Alternative Names
ATG21 antibody; CVT21 antibody; HSV1 antibody; MAI1 antibody; YPL100W antibody; Autophagy-related protein 21 antibody; Cytoplasm to vacuole transport protein 21 antibody; Homologous with SVP1 protein 1 antibody; Maturation of proaminopeptidase I protein 1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG21 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for cytoplasm to vacuole transport (Cvt) vesicles formation and mitophagy. Involved in binding of phosphatidylethanolamine to ATG8 and in recruitment of ATG8 and ATG5 to the pre-autophagosomal structure. Protects ATG8 from ARG4-mediated cleavage. Essential for maturation of proaminopeptidase I.
Gene References into Functions
  1. Atg21 is involved in the recruitment of membrane to the pre-autophagosomal structure. PMID: 15155809
  2. Mutated Atg21-(FTTGT) is inactive during the Cvt pathway but showed only partly reduced binding to PtdIns-phosphates, suggesting further lipid binding domains in Atg21 PMID: 16876790
Subcellular Location
Cytoplasm. Vacuole. Note=And perivacuolar punctate structures.
Protein Families
WD repeat SVP1 family
Database Links

KEGG: sce:YPL100W

STRING: 4932.YPL100W

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