ATG23 Antibody

Code CSB-PA933518XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG23 Polyclonal antibody
Uniprot No.
Target Names
ATG23
Alternative Names
ATG23 antibody; CVT23 antibody; YLR431CAutophagy-related protein 23 antibody; Cytoplasm to vacuole targeting protein 23 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG23 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for cytoplasm to vacuole transport (Cvt) vesicle formation and efficient autophagy. Plays a role in ATG protein retrieval from the pre-autophagosomal structure (PAS) and is especially required for autophagy-dependent cycling of ATG9. Plays also a role in regulation of filamentous growth.
Gene References into Functions
  1. Overexpression of Atg23 inhibits Atg9 trafficking to the phagophore assembly site. PMID: 25385507
Subcellular Location
Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Membrane; Peripheral membrane protein. Note=Found in pre-autophagosomal structure and other punctate structures. Correct localization of ATG23 to the membranes is strictly dependent of ATG9. Cycling through the pre-autophagosomal structure and correct location to other punctate structures is ATG1- and ATG13-dependent.
Protein Families
ATG23 family
Database Links

KEGG: sce:YLR431C

STRING: 4932.YLR431C

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