Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG9 Polyclonal antibody

Uniprot No.

Q12142

Target Names

ATG9

Alternative Names

ATG9 antibody; APG9 antibody; AUT9 antibody; CVT7 antibody; YDL149W antibody; D1560Autophagy-related protein 9 antibody; Cytoplasm to vacuole targeting protein 7 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG9 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Phospholipid scramblase involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Required for mitophagy. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. Recruits vesicle-tethering proteins TRS85 and YPT1 to the autophagosome formation site. Recruits also ATG23 and ATG8 to the PAS.

Gene References into Functions

Atg9, Stp4, and Gvp36 are putative new regulators of sphingolipid homeostasis in Saccharomyces cerevisiae. PMID: 28152593
This study identified Pho23 as a master transcriptional repressor for autophagy that regulates the frequency of autophagosome formation through its negative regulation of ATG9. PMID: 24881874
PAS localization of Atg9 was normal in atg27(DeltaYSAV) cells. PMID: 25557545
ATG9 trafficking to phagophore assembly site depends on ATG23 and ATG27 stochiometry. PMID: 25385507
TRAPPIII(trs85) is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy. PMID: 23986483
Data show the PtdIns 4-kinase Pik1 is involved in Atg9 trafficking through the Golgi and is involved in both nonselective and selective types of autophagy, whereas the PtdIns4P 5-kinase Mss4 is involved in mitophagy but not nonselective autophagy. PMID: 22977244
It was shown that that the vast majority of Atg9 existed on cytoplasmic mobile vesicles that were derived from the Golgi apparatus in a process involving Atg23 and Atg27. Only a few Atg9 vesicles were needed for a single round of forming autophagosomes. PMID: 22826123
Data show that membrane that contains Atg9 is delivered to the autophagosome from the Golgi-endosomal system rather than from the ER or mitochondria. PMID: 20861302
The role of Atg9 trafficking in yeast and mammalian autophagy, is summarised. PMID: 20083107
A different category of mutants that blocks Atg9 sorting from mitochondria, has been characterised. PMID: 16787937
we demonstrate that Atg9 shuttles between the preautophagosomal structure and mitochondria PMID: 16874040
Atg27 is required for Atg9 cycling and shuttles between the pre-autophagosomal structure, mitochondria, and the Golgi complex. PMID: 17135291
model proposed for Atg9 cycling suggests pool of Atg11 mediates anterograde transport of Atg9 to the pre-autophagosomal structure/phagophore assembly site along the actin cytoskeleton PMID: 17329962
Results support a model in which Atg9 multimerization facilitates membrane flow to the phagophore assembly site for phagophore formation. PMID: 18829864
Atg9 can both promote lipid transport from the membrane origins to the phagophore assembly site, and also help assemble an intact phagophore membrane PMID: 19182520

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Subcellular Location

Preautophagosomal structure membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein.

Protein Families

ATG9 family

Database Links

KEGG: sce:YDL149W

STRING: 4932.YDL149W

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ATG9 Antibody

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