atoS Antibody

Code CSB-PA312324XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) atoS Polyclonal antibody
Uniprot No.
Target Names
atoS
Alternative Names
atoS; b2219; JW2213; Signal transduction histidine-protein kinase AtoS
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
A synthesized peptide derived from Escherichia coli (strain K12) atoS protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, Dot blot (guaranteed specific to the immunizing peptide)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;
Quality Guarantee
1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Member of the two-component regulatory system AtoS/AtoC. In the presence of acetoacetate, AtoS/AtoC stimulates the expression of the atoDAEB operon, leading to short chain fatty acid catabolism and activation of the poly-(R)-3-hydroxybutyrate (cPHB) biosynthetic pathway. Also induces the operon in response to spermidine. Involved in the regulation of motility and chemotaxis, via transcriptional induction of the flagellar regulon. AtoS is a membrane-associated kinase that phosphorylates and activates AtoC in response to environmental signals.
Gene References into Functions
  1. AtoS is a membrane protein which can autophosphorylate & then transfer that phosphoryl group to AtoC. Gene products of atoS & atoC constitute a 2-component signal transduction system, with some unusual properties, involved in regulation of atoDAEB operon. PMID: 16153782
Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Database Links
CUSABIO guaranteed quality
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