atpB Antibody

Code CSB-PA015070XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) atpB Polyclonal antibody
Uniprot No.
Target Names
atpB
Alternative Names
atpB; papD; uncB; b3738; JW3716; ATP synthase subunit a; ATP synthase F0 sector subunit a; F-ATPase subunit 6
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) atpB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Key component of the proton channel; it plays a direct role in the translocation of protons across the membrane.
Gene References into Functions
  1. Interaction with monomeric subunit c drives insertion of ATP synthase subunit a into the membrane and primes a-c complex formation PMID: 21900248
  2. NMR assignments for the subunit a of the E. coli ATP synthase PMID: 15213458
  3. Data indicate that both the N- and C-terminal domains of PapD must fold into a native conformation prior to the formation of domain-domain interactions. PMID: 15504040
  4. molecular probes defined five transmembrane helices, the orientation of the protein in the membrane, and information about the relative exposure of the loops connecting these helices PMID: 16691481
  5. helices fold back on themselves in a chloroform-methanol-H2O (4:4:1) solvent mixture and then pack at an angle such that the cytoplasmic ends of the polypeptide backbone are significantly displaced from each other. PMID: 18178144

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Subcellular Location
Cell inner membrane; Multi-pass membrane protein.
Protein Families
ATPase A chain family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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