atpA Antibody

Code CSB-PA002344XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) atpA Polyclonal antibody
Uniprot No.
Target Names
atpA
Alternative Names
atpA antibody; papA antibody; uncA antibody; b3734 antibody; JW3712ATP synthase subunit alpha antibody; EC 7.1.2.2 antibody; ATP synthase F1 sector subunit alpha antibody; F-ATPase subunit alpha antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) atpA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.
Gene References into Functions
  1. in the F1-ATPase of Escherichia coli, the epsilon- inhibited state forms after ATP hydrolysis, is distinct from the ADP-inhibited state, and responds dynamically to catalytic site ligands PMID: 23400782
  2. UPEC4030 strain could contain an unknown papA variant or the novel genotype. PMID: 19069657
  3. The P pilus usher PapC R652 is important for high-affinity binding to the adhesin whereas F21 is important for limiting affinity for the PapA major rod subunit. PMID: 20199591
  4. Cys substitutions were introduced into two different transmembrane helices (TMHs) of subunit a, and the proximity of the thiol side chains was tested via attempted oxidative cross-linking to form the disulfide bond. PMID: 17035244
  5. During assembly of ATP synthase, alpha/delta subunit interaction is an important facet of proton pumping stator subunit structure and function. PMID: 17176112

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Subcellular Location
Cell inner membrane; Peripheral membrane protein.
Protein Families
ATPase alpha/beta chains family
Database Links
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