AUR1 Antibody

Code CSB-PA878654ZA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AUR1 Polyclonal antibody
Uniprot No.
Target Names
AUR1
Alternative Names
AUR1 antibody; At4g32830 antibody; T16I18.40Serine/threonine-protein kinase Aurora-1 antibody; AtAur1 antibody; EC 2.7.11.1 antibody; Aurora-like kinase 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AUR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Phosphorylates specifically 'Ser-10' of histone H3 in vitro and colocalizes with phosphorylated histone H3 during mitosis. Associates with cytoskeletal structures that are necessary for cytokinesis and with the microtubule spindle. Colocalizes also with gamma-tubulin and function in microtubule organizing centers (MTOCs). In contrast with the mammalian B-type Aurora, AUR1 has no kinase activity toward 'Ser-28' of histone H3.
Gene References into Functions
  1. a central role for AUR1 kinase in regulating formative division plane orientation throughout development PMID: 22045917
  2. AtAurora1 kinase may function to phosphorylate substrates that are critical to the spatiotemporal regulation of acentrosomal microtubule formation and organization. PMID: 22150830
  3. Aurora kinase AtAUR1 localized at the nuclear membrane in interphase and located in mitotic spindles and cell plates during cell division. PMID: 16028112
  4. AtAurora1 in vivo kinase activity was inhibited by hesperadin which also inhibited histone H3S10 phosphorylation. PMID: 19582900
Subcellular Location
Nucleus membrane. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, phragmoplast. Note=Nuclear membrane in interphase cells, spindle poles at prophase, mitotic spindle from metaphase to telophase and equatorial cell plate at telophase.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
Tissue Specificity
Abundant in roots, flowers and flower buds, low or absent in expanded leaves, stems and siliques.
Database Links

KEGG: ath:AT4G32830

STRING: 3702.AT4G32830.1

UniGene: At.24046

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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