AVP1 Antibody

Code CSB-PA330080XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AVP1 Polyclonal antibody
Uniprot No.
Target Names
AVP1
Alternative Names
AVP1 antibody; AVP antibody; AVP-3 antibody; AVP3 antibody; At1g15690 antibody; F7H2.3 antibody; Pyrophosphate-energized vacuolar membrane proton pump 1 antibody; EC 7.1.3.1 antibody; Pyrophosphate-energized inorganic pyrophosphatase 1 antibody; H(+)-PPase 1 antibody; Vacuolar proton pyrophosphatase 1 antibody; Vacuolar proton pyrophosphatase 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AVP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Contributes to the transtonoplast (from cytosol to vacuole lumen) H(+)-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H(+)-ATPase on the same membrane. In addition, facilitates auxin transport by modulating apoplastic pH and regulates auxin-mediated developmental processes. Confers tolerance to NaCl and to drought by increasing ion retention.
Gene References into Functions
  1. Transgenic Arabidopsis plants expressing both AVP1 and PP2A-C5 at relatively high levels were identified and analyzed. These plants displayed enhanced tolerance to NaCl compared to either AVP1 or PP2A-C5 overexpressing plants. PMID: 30080613
  2. AVP1 role in phloem loading and transport PMID: 26530315
  3. AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function. PMID: 25681328
  4. AVP1DOX plants produced 25% more marketable ripened fruit per plant under P-deficient conditions compared with the controls. Further, under low phosphate conditions, they displayed increased phosphate transport from leaf (source) to fruit (sink). PMID: 24723407
  5. The expression of AVP1 increases the shoot biomass of barley in saline and nonsaline conditions. It is shown that the expression of AVP1 in transgenic barley improves the grain yield per plant of this cereal crop when grown in a high-salinity field. PMID: 24261956
  6. Results show that transgenic plants expressing higher levels of AVP1 transcripts in sugarcane are able to withstand salt and drought stress regimes. PMID: 24002911
  7. The characterization of romaine lettuce (Lactuca sativa 'Conquistador') plants engineered to overexpress an intragenic gain-of-function allele of the type I proton translocating pyrophosphatase (H(+)-PPase) of Arabidopsis, is reported. PMID: 23307651
  8. Hydrolysis of cytosolic PPi, rather than vacuolar acidification, is the major function of AVP1/FUGU5 in planta. Plant cells optimize their metabolic function by eliminating PPi in the cytosol for efficient postembryonic heterotrophic growth. PMID: 21862707
  9. Heightened expression of AVP1 enhances pyrophosphate-dependent proton pump activity, salt tolerance, ion vacuolar sequestration, K+ uptake capacity, root hair development, osmotic responses, and PMATPase hydrolytic and proton pumping activities. PMID: 22195582
  10. reported that the H+-pyrophosphatase, AVP1, controls auxin transport and consequently auxin-dependent development [AVP1] PMID: 16210544

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Subcellular Location
Vacuole membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Mostly vacuolar, tonoplast. Also present in endosomes and plasma membrane.
Protein Families
H(+)-translocating pyrophosphatase (TC 3.A.10) family, K(+)-stimulated subfamily
Tissue Specificity
Ubiquitous (at protein level). Mostly expressed in vascular tissues, meristems and root pericycle.
Database Links

KEGG: ath:AT1G15690

STRING: 3702.AT1G15690.1

UniGene: At.21942

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