AXL2 Antibody

Code CSB-PA336513XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) AXL2 Polyclonal antibody
Uniprot No.
Target Names
AXL2
Alternative Names
AXL2 antibody; BUD10 antibody; SRO4 antibody; YIL140WAxial budding pattern protein 2 antibody; Bud site selection protein 10 antibody; Suppressor of RHO3 protein 4 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) AXL2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Required for haploid cells axial budding pattern. Acts as an anchor to help direct new growth components and/or polarity establishment components like the BUD5 GTP/GDP exchange factor to localize at the cortical axial budding site. Regulates septin organization in late G1 independently of its role in polarity-axis determination.
Gene References into Functions
  1. These results suggest that Axl2p plays sequential and distinct roles in the regulation of cellular morphogenesis in yeast cell cycle. PMID: 17460121
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Note=In small buds, localizes to incipient bud sites, emerging buds and to the bud periphery. In large buds, localizes as a ring at the bud neck. Requires ERV14 to be efficiently delivered to the cell surface. Recruitment to the bud neck after S/G2 phase of the cell cycle depends on BUD3 and BUD4.
Database Links

KEGG: sce:YIL140W

STRING: 4932.YIL140W

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