AXR1 Antibody

Datasheet

Code	CSB-PA015411XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AXR1 Polyclonal antibody

Uniprot No.

P42744

Target Names

AXR1

Alternative Names

AXR1 antibody; At1g05180 antibody; YUP8H12.21 antibody; NEDD8-activating enzyme E1 regulatory subunit AXR1 antibody; Auxin-resistance protein AXR1 antibody; Protein AUXIN-RESISTANT 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AXR1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulatory subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates RUB1/NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1. Plays an important role in auxin response. Regulates the chromosomal localization of meiotic recombination by crossovers (COs) and subsequent synapsis, probably through the activation of a CRL4 complex. Required for E3-mediated protein degradation in response to auxin, jasmonic acid and cold stress. Required for the COP1-COP10-CSN-mediated repression of photomorphogenesis in the dark. May function redundantly with AXL1 in the RUB conjugating pathway. Seems not to be functionally equivalent to AXL1 in vivo.

Gene References into Functions

AXR1 and other neddylation enzymes are autoneddylated in an E2- and E3-independent manner in vitro and that AXR1 autoneddylation impairs NEDD8 E2 approximately thioester formation in the den1 mutant background in vivo. PMID: 28096463
SMAP1 is necessary for root meristem formation and normal embryo development in the axr1 background. PMID: 22576848
Data show taht root hair cell-specific overexpression of the influx transporter AUXIN-RESISTANT1 enhanced root hair length. PMID: 18156217

Subcellular Location

Nucleus.

Protein Families

Ubiquitin-activating E1 family, ULA1 subfamily

Tissue Specificity

Expressed in shoot, root and floral meristems, in vascular tissues of cotyledons and mature leaves, and in the stele of the root. Expressed at higher levels on the lower side of an emerging root during germination and at higher levels on the underside of

Database Links

KEGG: ath:AT1G05180

STRING: 3702.AT1G05180.1

UniGene: At.10217

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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AXR1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background