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aca1 Antibody

Datasheet
Code CSB-PA116019XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) aca1 Polyclonal antibody
Uniprot No.
Q09927
Target Names
aca1
Alternative Names
aca1 antibody; ppr1 antibody; SPAC21E11.04N-acetyltransferase aca1 antibody; EC 2.3.1.271; antibody; S)-1-pyrroline-5-carboxylate acetyltransferase antibody; L-azetidine-2-carboxylate acetyltransferase antibody; AZC acetyltransferase antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) aca1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
N-acetyltransferase involved in oxidative stress resistance. Acetylates the toxic proline metabolism intermediate (S)-1-pyrroline-5-carboxylate (P5C), or more likely its spontaneously forming tautomer glutamate-5-semialdehyde (GSA) into N-acetyl-GSA for arginine synthesis in the mitochondria. P5C has been shown to increase the levels of reactive oxigen species (ROS) in the cell by inhibiting the function of the respiratory chain in the mitochondria. The enzyme is able to reduce intracellular ROS levels under P5C-induced oxidative stress and protects cells from damage by oxidative stress. Also acetylates and thereby detoxifies the proline analog azetidine-2-carboxylate (AZC), however it is unlikely that AZC is a natural substrate as it occurs only in plants belonging to the Lilaceae family.
Subcellular Location
Cytoplasm. Mitochondrion.
Protein Families
Acetyltransferase family
Database Links
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