AZF2 Antibody

Code CSB-PA794265XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AZF2 Polyclonal antibody
Uniprot No.
Target Names
AZF2
Alternative Names
AZF2 antibody; ZF2 antibody; At3g19580 antibody; MMB12.27 antibody; MMB12.4Zinc finger protein AZF2 antibody; Zinc-finger protein 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AZF2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional repressor involved in the inhibition of plant growth under abiotic stress conditions. Can repress the expression of various genes, including osmotic stress and abscisic acid-repressive genes and auxin-inducible genes, by binding to their promoter regions in a DNA sequence-specific manner. Acts as a negative regulator of abscisic acid (ABA) signaling during seed germination. Probably involved in jasmonate (JA) early signaling response. May regulate the expression of the JA biosynthesis gene LOX3 and control the expression of TIFY10A/JAZ1, a key repressor in the JA signaling cascade. May act as a positive regulator of leaf senescence. Has been identified as a suppressor of the deficiency of yeast snf4 mutant to grow on non-fermentable carbon source.
Gene References into Functions
  1. Transcript regulation of AtbZIP9 and AtAFZ2 during germination was compatible with the proposed role in transcriptional regulation of AtTrxo1. Transient over-expression of AtbZIP9 and AtAZF2 in Nicotiana benthamiana leaves demonstrated an activation effect of AtbZIP9 and a repressor effect of AtAZF2 on AtTrxo1 promoter-driven reporter expression. PMID: 28184497
  2. positive regulator of leaf senescence PMID: 22709441
  3. AZF1 and AZF2 function as transcriptional repressors involved in the inhibition of plant growth under abiotic stress conditions. PMID: 21852415
  4. Results indicate that AZF2 is a negative regulator of ABA signaling in seeds. PMID: 20619483
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in roots, radicles, cotyledons, hypocotyls, leaf veins, stems, sepals, petals, stamens, placenta, funiculi and maturated seeds.
Database Links

KEGG: ath:AT3G19580

STRING: 3702.AT3G19580.1

UniGene: At.23023

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