B4GALT1 Antibody

Code CSB-PA002513ZA01BO
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Product Details

Full Product Name
Rabbit anti-Bos taurus (Bovine) B4GALT1 Polyclonal antibody
Uniprot No.
Target Names
B4GALT1
Alternative Names
B4GALT1 antibody; GALT antibody; GGTB2Beta-1,4-galactosyltransferase 1 antibody; Beta-1,4-GalTase 1 antibody; Beta4Gal-T1 antibody; b4Gal-T1 antibody; EC 2.4.1.- antibody; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase antibody; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase antibody; EC 2.4.1.38 antibody; Lactose synthase A protein antibody; EC 2.4.1.22 antibody; N-acetyllactosamine synthase antibody; EC 2.4.1.90 antibody; Nal synthase antibody; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1 antibody; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1) [Cleaved into: Processed beta-1,4-galactosyltransferase 1] antibody
Raised in
Rabbit
Species Reactivity
Bos taurus
Immunogen
Recombinant Bos taurus B4GALT1 protein
Immunogen Species
Bos taurus (Bovine)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.; The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. The secreted form is responsible for the synthesis of complex-type to N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Gene References into Functions
  1. Hypothesized that engineering B4GALT1 to block cleavage and secretion would enhance its retention and its function; to test this hypothesis, replaced CTS domains of bovine B4GALT1 with those from human FUT7, which is not cleaved and secreted. PMID: 25462875
  2. the beta4galt1 gene is potentially associated with milk production traits in dairy cows and should be considered for further studies on genetics of the milk production traits. PMID: 22311019
  3. Crystal structure of mutant Met344His beta4Gal-T1 defined at 2.3 angstrom resolution reveals that the N-acetylglucosamine residue at nonreducing end of chitobiose makes extensive hydrophobic interactions with highly conserved tyrosine-286 of beta4Gal-T1. PMID: 15449940
Subcellular Location
[Isoform Long]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium.; [Isoform Short]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 7 family
Tissue Specificity
Detected in milk (at protein level).
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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