BAM1 Antibody

Code CSB-PA525069XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BAM1 Polyclonal antibody
Uniprot No.
Target Names
BAM1
Alternative Names
BAM1 antibody; At5g65700 antibody; F6H11.170 antibody; MPA24.5Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM1 antibody; EC 2.7.11.1 antibody; Protein BARELY ANY MERISTEM 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BAM1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Necessary for male gametophyte development, as well as ovule specification and function. Involved in cell-cell communication process required during early anther development, and regulating cell division and differentiation to organize cell layers. Required for the development of high-ordered vascular strands within the leaf and a correlated control of leaf shape, size and symmetry. May regulate the CLV1-dependent CLV3-mediated signaling in meristems maintenance.
Gene References into Functions
  1. Driven by a constitutive CaMV 35S promoter, a truncated BAM protein (BAMDelta) that lacks either the signal peptide or the cytoplasmic kinase domain could cause defective shoot apical meristem maintenance, which phenotypically resembled the triple bam mutant. Such a dominant-negative effect could also be achieved when the same transgene was driven by the native AtBAM1 promoter. PMID: 29606214
  2. BAM1 is an element required for the cell-to-cell spread of RNAi. PMID: 29363594
  3. Sodium nitroprusside, which releases NO, did not affect BAM1 activity. After treatment of recombinant BAM1 with GSNO, BAM1 was largely unaffected. A BAM1 mutant resembling BAM3 was sensitive to GSNO inhibition. PMID: 29309132
  4. Study demonstrate that abscisic acid controls the activity of BAM1 and AMY3 in leaves under osmotic stress through the AREB/ABF-SnRK2 kinase-signaling pathway. We propose that differential regulation and isoform subfunctionalization define starch-adaptive plasticity, ensuring an optimal carbon supply for continued growth under an ever-changing environment. PMID: 27436713
  5. BAM1 directly interacts with the small peptide ligand CLE9. PMID: 22321211
  6. Thioredoxin-regulated BAM1 activates a starch degradation pathway in illuminated mesophyll cells upon osmotic stress. PMID: 20876336
  7. BAM1 regulate stem cell specification and organ development through complex interactions with CLAVATA signaling PMID: 18780746
  8. Analysis of BAM1 and BAM2 reveal a cell-cell communication process important for early anther development, including aspects of cell division and differentiation [BAM1] PMID: 16751349

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Expressed in seedlings, roots, leaves, inflorescences, flowers and siliques.
Database Links

KEGG: ath:AT5G65700

STRING: 3702.AT5G65700.1

UniGene: At.1921

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