brd-1 Antibody

Code CSB-PA630333XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans brd-1 Polyclonal antibody
Uniprot No.
Target Names
brd-1
Alternative Names
brd-1 antibody; K04C2.4BRCA1-associated RING domain protein 1 antibody; BARD1 antibody; Ce-BRD-1 antibody; Cebrd-1 antibody; EC 2.3.2.27 antibody; RING-type E3 ubiquitin transferase BARD1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans brd-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Constituent of the CeBCD complex that possesses E3 ubiquitin-protein ligase activity. When bound to chromatin, the brc-1-brd-1 heterodimer within the CeBCD complex is inactive during normal conditions, but in response to DNA damage, the brc-1-brd-1 heterodimer associates with other proteins such as the recombinase rad-51 or the E2-ubiquitin-conjugating enzyme let-70, which activate the CeBCD complex as an E3-ubiquitin ligase. Moreover, association between the brc-1-brd-1 heterodimer and rad-51 and let-70, probably requires DNA checkpoint proteins such as atl-1 and mre-11 in order to induce ubiquitination at DNA damage sites. To this end, the brc-1-brd-1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a role in triggering cellular responses at damage sites in response to DNA damage that may be induced by ionizing radiation for example. In particular, protects against chromosome non-disjunction and nuclear fragmentation during meiotic double-strand break repair to ensure sister chromatid recombination and aid chromosome stability.
Subcellular Location
Cytoplasm. Nucleus. Chromosome.
Database Links

KEGG: cel:CELE_K04C2.4

STRING: 6239.K04C2.4.3

UniGene: Cel.5319

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