BASL Antibody

Code CSB-PA263602XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BASL Polyclonal antibody
Uniprot No.
Target Names
BASL
Alternative Names
BASL antibody; At5g60880 antibody; MAE1.13Protein BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BASL protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Regulates asymmetric cell division (ACD), especially in stomatal-lineage cells, probably by modulating accumulation and subcellular polarization of POLAR and SPCH. Mediates an attenuation of MAPK signaling upon polarization of POLAR and ASK7/BIN2 in stomatal lineage ground cells (SLGCs) undergoing ACD, and relieves BIN2 inhibition of SPCH in the nucleus. When phosphorylated, functions as a scaffold and recruits the MAPKKK YODA, MPK3 and MPK6 to spatially reorganize the MAPK signaling pathway at the cortex of cells undergoing ACD. Cortical polarization leads to elevated nuclear MPK6 signaling and lowered SPCH abundance in one of the two daughter cells, thus differentiating the two daughter cells after ACD.
Gene References into Functions
  1. fine-scale dissection of the subdomains of polarity in stomatal asymmetric cell division PMID: 27422992
  2. a positive-feedback loop between BASL and the MAPK pathway constitutes a polarity module at the cortex. Cortical localization of BASL requires phosphorylation mediated by MPK3/6. PMID: 25843888
  3. Study identified BASL, a novel regulator of asymmetric divisions in Arabidopsis. PMID: 19523675
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, cell cortex. Cell membrane; Peripheral membrane protein.
Tissue Specificity
Mostly expressed in stomatal lineage cells including asymmetrically dividing meristemoid mother cells (MMCs) and meristemoids, and, at lower levels, in their sisters. Also present in vasculature. Expressed at low levels in the epidermal pavement cells.
Database Links

KEGG: ath:AT5G60880

STRING: 3702.AT5G60880.1

UniGene: At.51260

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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