Pi-actitoxin-Ael2b Antibody

Code CSB-PA351639XA01FRU
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Product Details

Uniprot No.
Alternative Names
Pi-actitoxin-Ael2b antibody; Pi-AITX-Ael2b antibody; Toxin APETx2 antibody
Raised in
Rabbit
Species Reactivity
Anthopleura elegantissima (Green aggregating anemone) (Actinia elegantissima)
Immunogen
Recombinant Anthopleura elegantissima (Green aggregating anemone) (Actinia elegantissima) Pi-actitoxin-Ael2b protein
Immunogen Species
Anthopleura elegantissima (Green aggregating anemone) (Actinia elegantissima)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
This toxin potently blocks acid-sensing ion channel ASIC3 homotrimers and heterotrimers containing ASIC3 (composed with isoforms of ASIC1 and ASIC2). It also weakly inhibits potassium channels, and sodium channels. On homomeric ASIC3, this protein shows IC(50)=57-87 nM on rat, 37.3 nM on mouse and 175 nM on human channels. The blockade is rapid and reversible. On heterotrimeric forms, the toxin is less potent (IC(50)=117 nM on rat ASIC2b-ASIC3 channel, 900 nM on rat ASIC1b-ASIC3, and 2 uM on rat ASIC1a-ASIC3). It weakly inhibits Kv3.4/KCNC4 potassium channels (3 uM of the toxin inhibits 38% of Kv3.4 current). It reversibly and voltage-dependently inhibits hKv11.1/KCNH2/ERG1 potassium channels (IC(50)=1.21 uM), inhibiting both peak and tail currents without action on channel inactivation. It weakly inhibits rNav1.2/SCN2A (EC(50)=114 nM), rNav1.6/SCN8A current (17% at 1 uM of the toxin) and Nav1.8/SCN10A (IC(50)=6.6 uM on human channels expressed in oocytes, EC(50)=55 nM on rat channels expressed in oocytes, and 2.6 uM on rat channels in DRG neurons). It may act on sodium channels by binding at site 1 or close by, when the pore is in an open configuration. In vivo, central injection does not induce neurotoxin symptoms in mice even after 24 hours. However, it abolishes acid-induced pain in rats.
Subcellular Location
Secreted. Nematocyst.
Protein Families
Sea anemone type 3 (BDS) potassium channel toxin family
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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