BIOF Antibody

Code CSB-PA104859XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BIOF Polyclonal antibody
Uniprot No.
Target Names
BIOF
Alternative Names
BIOF antibody; BIO4 antibody; At5g04620 antibody; T32M21.2208-amino-7-oxononanoate synthase antibody; AONS antibody; EC 2.3.1.47 antibody; 7-keto-8-amino-pelargonic acid synthase antibody; 7-KAP synthase antibody; KAPA synthase antibody; 8-amino-7-ketopelargonate synthase antibody; Biotin synthase 4 antibody; Biotin synthase F antibody; AtbioF antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BIOF protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. Required for the biosynthesis of D-biotin that prevents light-mediated cell death and modulates defense gene expression, probably by avoiding hydrogen peroxide H(2)O(2) accumulation.
Gene References into Functions
  1. Data show that the bio4-1 plants (mutant of BIO4, At5g04620) exhibited massive accumulation of hydrogen peroxide and constitutive up-regulation of a number of genes that are diagnostic for defense and reactive oxygen species signaling. PMID: 22126457
Subcellular Location
Cytoplasm, cytosol. Peroxisome.
Protein Families
Class-II pyridoxal-phosphate-dependent aminotransferase family, BioF subfamily
Database Links

KEGG: ath:AT5G04620

STRING: 3702.AT5G04620.2

UniGene: At.46019

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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