BKI1 Antibody

Code CSB-PA884304XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BKI1 Polyclonal antibody
Uniprot No.
Target Names
BKI1
Alternative Names
BKI1 antibody; At5g42750 antibody; MJB21.13BRI1 kinase inhibitor 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BKI1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Negative regulator of brassinosteroid signaling. When associated to the membrane, limits the interaction of BRI1 with BAK1 by binding to the kinase-inactive form of BRI1.
Gene References into Functions
  1. PUB30 negatively regulates salt tolerance probably through regulating the degradation of BKI1 and brassinosteroids signaling in Arabidopsis. PMID: 28865087
  2. BRI1 KINASE INHIBITOR 1 (BKI1) is tyrosine phosphorylated in response to brassinosteroid perception. PMID: 21289069
  3. a BRI1-interacting protein, BKI1, a negative regulator of brassinosteroid signaling is reported; BKI1 is a substrate of BRI1 kinase & limits interaction of BRI1 with its proposed coreceptor, BAK1, suggesting that BKI1 prevents activation of BRI1 [BKI1] PMID: 16857903
Subcellular Location
Cell membrane. Cytoplasm. Note=Once phosphorylated following brassinosteroid treatment, displaced from the plasma membrane into the cytosol where it is inactive.
Tissue Specificity
Expressed in leaves, petioles, shoot apices, hypocotyls, roots and flowers.
Database Links

KEGG: ath:AT5G42750

STRING: 3702.AT5G42750.1

UniGene: At.30155

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