bolA Antibody

Code CSB-PA359608XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) bolA Polyclonal antibody
Uniprot No.
Target Names
bolA
Alternative Names
bolA antibody; b0435 antibody; JW5060DNA-binding transcriptional regulator BolA antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) bolA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcriptional regulator that plays an important role in general stress response. Has many effects on cell morphology, cell growth and cell division. Acts by regulating the transcription of many genes, including dacA (PBP-5), dacC (PBP-6), ampC and mreB. Probably involved in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions. Essential for normal cell morphology in stationary phase and under conditions of starvation. Also regulates a complex network of genes encoding proteins related to biofilm development, and negatively modulates flagellar biosynthesis and swimming capacity. Could be a motile/adhesive transcriptional switch, specifically involved in the transition between the planktonic and the attachment stage of biofilm formation. Overexpression produces round cell shape, impairs cell growth rate and induces biofilm development.
Gene References into Functions
  1. study unravelled a direct connection between the bolA morphogene and the signaling molecule c-di-GMP; show the important cross-talk that occurs between these two molecular regulators during the transition between the motile/planktonic and adhesive/sessile lifestyles in E. coli PMID: 28928205
  2. In the presence of bolA, E. coli cells were able to attach to the stainless steel and silicone. PMID: 27842452
  3. These data show that BolA is a new bacterial transcription factor that modulates the switch from a planktonic to a sessile lifestyle. PMID: 25691594
  4. Authors summarize current knowledge on the role of BolA, mainly in E. coli, and discuss its implication in global regulation in relation to stress. [Review] PMID: 25038865
  5. E. coli K-12 MG1655, rpoS, and bolA mutant strains were used and gene expression was studied. Results show that both genes contribute to the ability to respond and adapt in response to various types of stresses PMID: 21630090
  6. alterations in cell morphology induced by bolA seem to be mediated by a complex pathway that integrates PBP5, PBP6, MreB, and probably other regulators of cell morphology/elongation. PMID: 19111750

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Protein Families
BolA/IbaG family
Database Links
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