BON1 Antibody

Code CSB-PA821975XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BON1 Polyclonal antibody
Uniprot No.
Target Names
BON1
Alternative Names
BON1 antibody; CPN1 antibody; At5g61900 antibody; K22G18.2Protein BONZAI 1 antibody; COPINE 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana BON1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Negative regulator of cell death and defense responses. Negative regulator of several R genes, including SNC1. May have effects in promoting growth and development. May function in membrane trafficking and in fusion of vesicles with plasma membrane at low temperature. Exhibits calcium-dependent phospholipid binding properties.
Gene References into Functions
  1. Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis. PMID: 20634289
  2. Overexpression of BAP1 or BAP2 with their partner BON1 inhibits programmed cell death induced by pathogens, the proapoptotic gene BAX, and superoxide-generating paraquat. PMID: 17631528
  3. Promoter deletion analysis identified a 280 bp segment of the BON1/CPN1 promoter as being required for pathogen-induced gene expression; the same promoter region was also required for calcium ionophore-induced gene expression PMID: 18855102
  4. BON1 and BON3 represses the transcript accumulation and/or protein activities of multiple resistance (R)-like genes. PMID: 19522566
Subcellular Location
Cell membrane; Lipid-anchor.
Protein Families
Copine family
Tissue Specificity
Expressed in roots and flowers and, at higher levels, in leaves and stems. Strongly expressed in growing tissues. Not detected in green siliques.
Database Links

KEGG: ath:AT5G61900

STRING: 3702.AT5G61910.4

UniGene: At.20314

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