HUB1 Antibody

Code CSB-PA823316XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HUB1 Polyclonal antibody
Uniprot No.
Target Names
HUB1
Alternative Names
HUB1 antibody; BRE1A antibody; RDO4 antibody; At2g44950/At2g44960 antibody; T13E15.24/T13E15.23 antibody; T14P1.25/T14P1.24 antibody; E3 ubiquitin-protein ligase BRE1-like 1 antibody; AtBRE1 antibody; EC 2.3.2.27 antibody; Protein HISTONE MONOUBIQUITINATION 1 antibody; AtHUB1 antibody; Protein REDUCED DORMANCY 4 antibody; RING-type E3 ubiquitin transferase BRE1-like 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HUB1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
E3 ubiquitin-protein ligase that monoubiquitinates H2B to form H2BK143ub1. H2BK143ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and maybe H3K79me. It thereby plays a central role in histone code and gene regulation. Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBC2/RAD6. Required for the regulation of flowering time and defense against necrotrophic fungal pathogens. Involved in the control of seed dormancy and germination.
Gene References into Functions
  1. Two RING E3 ligases, HISTONE MONOUBIQUITINATION 1 and 2 (HUB1 and HUB2), play a role in the selective transcriptional activation of four cuticle biosynthesis genes in Arabidopsis thaliana. PMID: 24319075
  2. Data showed that parameters for plant fitness are altered in HUB1 mutant and overexpression lines, suggesting that the histone H2B monoubiquitination status affects plant fitness. PMID: 23073007
  3. Double mutants between hub1-1 and elongata (elo) showed an embryo-lethal phenotype, indicating a synergistic genetic interaction. PMID: 22762858
  4. role is proposed for chromatin remodeling in seed dormancy by H2B monoubiquitination through HUB1 and HUB2 PMID: 17329563
  5. Data show that HUB1 is a functional homolog of the human and yeast BRE1 proteins, adn suggest that it mediates gene activation and cell cycle regulation through chromatin modifications. PMID: 17329565
  6. HUB1 upregulates the expression of FLC, and interacts with UBC1 and UBC2 to repress flowering. PMID: 18980658
  7. Results describe the role of the Arabidopsis thaliana RING E3 ligase, HISTONE MONOUBIQUITINATION1 (HUB1) in disease resistance. PMID: 19286969

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Subcellular Location
Nucleus.
Protein Families
BRE1 family
Tissue Specificity
Ubiquitously expressed.
Database Links

KEGG: ath:AT2G44950

STRING: 3702.AT2G44950.1

UniGene: At.43266

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