BRE5 Antibody

Code CSB-PA345315XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BRE5 Polyclonal antibody
Uniprot No.
Target Names
BRE5
Alternative Names
BRE5 antibody; YNR051C antibody; N3465UBP3-associated protein BRE5 antibody; Brefeldin-A sensitivity protein 5 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BRE5 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Has a role in de-ubiquitination. In conjunction with UBP3, cleaves ubiquitin, leading to the subsequent mono-ubiquitination of sec23.
Gene References into Functions
  1. analysis of Ubp3 deubiquitinating enzyme interaction with its Bre5 cofactor PMID: 15955808
  2. Ubp3/Bre5 functions antagonistically with Bul1 in DNA damage responses, but not in its other cellular functions PMID: 17556048
  3. New insights into Ubp3 recognition by Bre5 and into protein recognition by NTF2-like domains. PMID: 17632125
  4. show that mature ribosomes are rapidly degraded by autophagy upon nutrient starvation in Saccharomyces cerevisiae, and that selective degradation of ribosomes requires catalytic activity of the Ubp3p/Bre5p ubiquitin protease. PMID: 18391941
Database Links

KEGG: sce:YNR051C

STRING: 4932.YNR051C

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