BRO1 Antibody

Code CSB-PA341773XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BRO1 Polyclonal antibody
Uniprot No.
Target Names
BRO1
Alternative Names
BRO1 antibody; ASI6 antibody; LPF2 antibody; NPI3 antibody; VPS31 antibody; YPL084WVacuolar-sorting protein BRO1 antibody; Amino acid sensor-independent protein 6 antibody; BCK1-like resistance to osmotic shock protein 1 antibody; BRO domain-containing protein 1 antibody; Nitrogen permease inactivating protein 3 antibody; Vacuolar protein-sorting-associated protein 31 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) BRO1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p-mitogen-activated protein kinase pathway.
Gene References into Functions
  1. Bro1 and other Bro1 family members function in parallel with ESCRT-0 to recognize and sort Ub-cargoes PMID: 23726974
Subcellular Location
Cytoplasm. Endosome.
Database Links

KEGG: sce:YPL084W

STRING: 4932.YPL084W

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