bub-1 Antibody

Datasheet

Code	CSB-PA877388XA01CXY
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans bub-1 Polyclonal antibody

Uniprot No.

Q21776

Target Names

bub-1

Alternative Names

bub-1 antibody; R06C7.8 antibody; Mitotic checkpoint serine/threonine-protein kinase bub-1 antibody; EC 2.7.11.1 antibody; Budding uninhibited by benzimidazole 1 antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans bub-1 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Serine/threonine-protein kinase essential for spindle-assembly checkpoint signaling. Plays a key role in the recruitment of the checkpoint proteins bub-3, mdf-1 and mdf-2 to unattached kinetochores. mdf-1 recruitment is independent of bub-1 kinase activity. Has a role in the correct kinetochore localization of the spindly-like protein spdl-1. In addition, during meiotic anaphase I, controls the recruitment of hcp-1/2 and klp-19 to the ring-shaped domain formed between chromosomes. Involved in chromosome alignment, chromosome homolog segregation and spindle assembly. In association with bub-3 at the kinetochore region of chromosomes, promotes the onset on anaphase independently from spindle checkpoint signaling and promotes the formation of stable end-on bipolar attachments of chromosomes. Plays a role in nuclear envelope breakdown. Required maternally during embryogenesis and in the zygote for the postembryonic development of several tissues including ventral cord neurons, gonad, intestine and seam cells.

Gene References into Functions

The BUB-1/BUB-3 complex promotes timely anaphase onset in Caenorhabditis elegans embryos. PMID: 25987605
An interaction between BUB-1 and MAD-1 is essential for spindle checkpoint signaling. PMID: 24567362
C. elegans bub-1 is required both maternally and zygotically PMID: 19526056

Subcellular Location

Cytoplasm, cell cortex. Nucleus. Chromosome, centromere, kinetochore.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily

Database Links

KEGG: cel:CELE_R06C7.8

STRING: 6239.R06C7.8

UniGene: Cel.17390

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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